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Image Search Results
Journal: bioRxiv
Article Title: Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5
doi: 10.1101/312546
Figure Lengend Snippet: All cells are HeLa cells. ( a ) The Golgi localization of different mutant forms of Arl5b. Cells transiently expressing Arl5b-GFP in QL, TN or wt form were fixed and endogenous Golgin-245 was stained. ( b ) Endogenous Arl5b localizes to the Golgi. HeLa cells were fixed and endogenous Arl5b and GS28 were co-stained. ( c,d ) Arl5b colocalizes with Lamtor1 at the endosome and lysosome. HeLa cells transiently co-expressing indicated GFP or mCherry-tagged proteins were imaged under live cell condition. ( e ) Lamtor1 localizes to the EE, LE and lysosome. Endogenous Lamtor1 was co-stained with exogenously expressed GFP-Rab7, endogenous EEA1 or Lamp1, respectively. In c-e , the boxed region was enlarged in the upper right corner to show the colocalization at puncta (denoted by arrows). Scale bar, 10 μm.
Article Snippet: The following antibodies were from
Techniques: Mutagenesis, Expressing, Staining